小鼠胰高血糖素即用型免疫组化试剂盒-分析方法-资讯-生物在线

小鼠胰高血糖素即用型免疫组化试剂盒

英文名称

Mouse Glucagon Ready-To-Use IHC Kit

中文名称

小鼠胰高血糖素即用型免疫组化试剂盒

别名

GCG; Glicentin related polypeptide; GLP 1; GLP 2; GLP1; GLP2; Glucagon like peptide 1; Glucagon like peptide 2; Glucagon precursor; GRPP; GLUC_HUMAN.

种属

Mouse

应用范围

Immunohistochemistry

适用样品基质

PFPE tissue

描述

IHC0104 is a ready-to-use IHC kit for staining of Glucagon. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

保存条件

Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.

注意事项

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍

产品组分及规格

编号	组分	规格	浓度	储存
1	PBS缓冲液（干粉）	2 L×2	20x	室温
2	抗原修复缓冲液	20 ml	100x	2-8℃
3	内源性过氧化物酶阻断剂	3 ml	RTU	2-8℃
4	封闭工作液	3 ml	RTU	2-8℃
5	一抗（Mouse Glucagon Rabbit pAb）	6 ml	RTU	2-8℃
6	二抗（HRP-Goat anti-Rabbit IgG pAb）	6 ml	RTU	2-8℃
7	DAB kit（20×）显色液	0.3 ml	RTU	-20℃
8	DAB kit（20×）稀释液	0.3 ml	RTU	-20℃
9	复染试剂	5 ml	RTU	室温
10	封片剂	5 ml	RTU	室温
11	对照切片（小鼠胰腺）	1张	RTU	室温
12	说明书	1份

背景

Glucagon is a 29-residue polypeptide hormone (MW 3482), produced in the pancreas. A related hormone, enteroglucagon (or oxyntomodulin), which is produced in the mucosa of the small and large intestine, consists of the 29 amino acid sequence of pancreatic glucagon extended by 8 additional residues at the C-terminus. The biological activities of pancreatic glucagon include glycogenolysis, lipolysis, gluconeogenesis, and ketogensis, which are antagonistic effects to those of insulin action, thus leading to increased blood glucose levels. Immunocytochemical studies have revealed the presence of pancreatic glucagon inside the A or alpha cells, which constitute 15-20% of the islet cell population. These cells are located preferentially at the periphery of the human pancreatic islets. Pathological manifestations of the glucagon-type peptide residue almost exclusively with the exsistence of tumors or glucagonomas, as no states of glucagon-cell deficiency or hyperplasia have been identified. Glucagon-specific antibodies would prove useful as a cell and tumor markers applying immunohistochemical techniques, and as an analytical tool in qualification of the hormone.

石蜡包埋组织的免疫组织化学方案

1. 脱蜡水化

石蜡切片置于新鲜二甲苯中浸泡脱蜡3次，每次15 min；依次置于不同浓度（100%、95%、90%、80%、70%）乙醇浸泡各5 min，再置于蒸馏水洗涤5 min，重复3次。

2. 抗原修复

沸水浴修复：将100×抗原修复缓冲液（试剂2）用蒸馏水稀释成1×抗原修复缓冲液，放入修复盒中并提前加热至95-100℃（注意盖好以防液体蒸发），然后将切片放入修复盒中，在沸水浴环境中保持外沸状态15 min，室温自然冷却；用PBS缓冲液（试剂1，将干粉溶解在2L蒸馏水中）清洗5 min，重复3次。

3. 阻断内源性过氧化物酶

用吸水纸吸去玻片上多余的液体，用免疫组化笔在组织周围画圈，加入2-4滴内源性过氧化物酶阻断剂（试剂3），室温下置于湿盒中孵育15 min，用PBS洗涤5 min，重复3次。

4. 血清封闭

用吸水纸吸去玻片上多余的液体，加入2-4

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