NFKBIA (phospho Y42) polyclonal antibody
产品名称: NFKBIA (phospho Y42) polyclonal antibody
英文名称: NFKBIA (phospho Y42) polyclonal antibody
产品编号: PAB7922
产品价格: null
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围:
亚诺法生技股份有限公司(Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of NFKBIA.
- Immunogen:
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y42 of human NFKBIA.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse, Rat
- Specificity:
- This peptide sequence has low homology to other IkB proteins, but does have some homology to unrelated proteins that may have a conserved tyrosine phosphorylation motif.
- Form:
- Liquid
- Storage Buffer:
- In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
- Storage Instruction:
- Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:2000)
Immunoprecipitation (5-10 ul)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- An atypical NF-kappa B-regulated pathway mediates phorbol ester-dependent heme oxygenase-1 gene activation in monocytes.
Naidu S, Wijayanti N, Santoso S, Kietzmann T, Immenschuh S.J Immunol. 2008 Sep 15;181(6):4113-23.
- 2.
- Epidermal growth factor (EGF) activates nuclear factor-kappaB through IkappaBalpha kinase-independent but EGF receptor-kinase dependent tyrosine 42 phosphorylation of IkappaBalpha.
Sethi G, Ahn KS, Chaturvedi MM, Aggarwal BB.Oncogene. 2007 Nov 15;26(52):7324-32. Epub 2007 May 28.
- 3.
- Regulation of COX-2 mediated signaling by alpha3 type IV noncollagenous domain in tumor angiogenesis.
Boosani CS, Mannam AP, Cosgrove D, Silva R, Hodivala-Dilke KM, Keshamouni VG, Sudhakar A.Blood. 2007 Aug 15;110(4):1168-77. Epub 2007 Apr 10.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of A-431 cells treated with pervanadate (1 mM) for 30 min. Blots were probed with NFKBIA (phospho Y42) polyclonal antibody (Cat # PAB7922). The latter antibodies were used in the presence of no blocking peptide (lane 1), phospho-NFKBIA (Tyr-42) peptide (lane 2), phospho-NFKBIA (Tyr-305) peptide (lane 3), or BSA conjugated to phospho-tyrosine (lane 4). Peptides and BSA-pTyr were used at 1 mg/ml.
- Entrez GeneID:
- 4792
- Gene Name:
- NFKBIA
- Gene Alias:
- IKBA,MAD-3,NFKBI
- Gene Description:
- nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
- Omim ID:
- 164008
- Gene Ontology:
- Hyperlink
- Gene Summary:
- NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM
- Other Designations:
- IkappaBalpha,nuclear factor of kappa light chain gene enhancer in B-cells
- Gene Pathway
- Adipocytokine signaling pathway
- Apoptosis
- B cell receptor signaling pathway
- Chemokine signaling pathway
- Chronic myeloid leukemia
- Epithelial cell signaling in Helicobacter pylori infection
- Neurotrophin signaling pathway
- Pathways in cancer
- Prostate cancer
- Small cell lung cancer
- T cell receptor signaling pathway
- Toll-like receptor signaling pathway
- Related Disease
- Adenocarcinoma
- Alcoholism
- Arthritis, Psoriatic
- Arthritis, Rheumatoid
- Asthma
- Asthma
- Atherosclerosis
- Behcet Syndrome
- Breast cancer
- Breast Neoplasms
- Bronchiolitis, Viral
- Calcinosis
- Carcinoma, Hepatocellular
- Carcinoma, Non-Small-Cell Lung
- Cardiovascular Diseases
- Carotid Stenosis
- Colorectal Neoplasms
- Connective Tissue Diseases
- Coronary Artery Disease