Synonyms:	OACP4 C(人胃癌细胞)
Background:	OACP4 C was established from a primary tumour located in gastric cardia of a 55 year-old Caucasian male. The patient showed lymph node involvement with pleural metastases. OACP4 C was found to be tumourigenic in NMRI (female nude mice or NOD-SCID mice. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis. Cultures derived from ECACC stocks of this cell line have been whole genome sequenced (Contino et al 2016) confirming the presence of many of the known mutations that drive oesophageal cancer
Species:	human (Homo sapiens)
Tissue:	Gastric cardia
Disease:	Gastric cardia adenocarcinoma,
Gender:	55 year-old Caucasian male
Morphology:	Pleiomorphic: both epithelial and fibroblastoid cells with cytoplasmic protrusions
Growth Mode:	Adherent
Doubling Time:	N/A
DNA Profile:	Amelogenin: X CSF1PO: 11 D13S317: 12 D16S539: 12 D5S818: 9 D7S820: 9,11 THO1: 9 TPOX: 11 vWA: 16 f Cell Line Authentication Service
Culture Medium:	RPMI-1640+10%FBS We strongly suggest to purchase the complete medium
Cryopreservation medium:	90%FBS+10%DMSO
Karyotype:	Aneuploid 53-57 chromosomes
Subculture Routine:	Split sub-confluent cultures (70-80%) up to 1:5 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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