NAE1 polyclonal antibody
产品名称： NAE1 polyclonal antibody
英文名称： NAE1 polyclonal antibody
产品编号： PAB2845
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of NAE1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human NAE1.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- APP-BP1 mediates APP-induced apoptosis and DNA synthesis and is increased in Alzheimer's disease brain.
Chen Y, Liu W, McPhie DL, Hassinger L, Neve RL.J Cell Biol. 2003 Oct 13;163(1):27-33.
- 2.
- ASPP2 inhibits APP-BP1-mediated NEDD8 conjugation to cullin-1 and decreases APP-BP1-induced cell proliferation and neuronal apoptosis.
Chen Y, Liu W, Naumovski L, Neve RL.J Neurochem. 2003 May;85(3):801-9.
- 3.
- Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8.
Walden H, Podgorski MS, Schulman BA.Nature. 2003 Mar 20;422(6929):330-4.
- Applications
- Western Blot (Tissue lysate)
- Western blot analysis of NAE1 polyclonal antibody (Cat # PAB2845) in mouse brain tissue lysates (35 ug/lane). NAE1 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
- Western Blot (Transfected lysate)
- Western blot analysis of NAE1 (arrow) using rabbit NAE1 polyclonal antibody (Cat # PAB2845). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NAE1 gene (Lane 2) (Origene Technologies).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human breast carcinoma reacted with NAE1 polyclonal antibody (Cat # PAB2845) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Entrez GeneID:
- 8883
- Protein Accession#:
- Q13564;NP_003896
- Gene Name:
- NAE1
- Gene Alias:
- A-116A10.1,APPBP1,HPP1,ula-1
- Gene Description:
- NEDD8 activating enzyme E1 subunit 1
- Omim ID:
- 603385
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
- Other Designations:
- NEDD8-activating enzyme E1 subunit,amyloid beta precursor protein binding protein 1, 59kDa,amyloid beta precursor protein-binding protein 1, 59kD,amyloid protein-binding protein 1,protooncogene protein 1
- Gene Pathway