SIAH1/SIAH2 monoclonal antibody, clone 8G7H12
产品名称： SIAH1/SIAH2 monoclonal antibody, clone 8G7H12
英文名称： SIAH1/SIAH2 monoclonal antibody, clone 8G7H12
产品编号： MAB2299
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
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- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against synthetic peptide of SIAH1/SIAH2.
- Immunogen:
- A synthetic peptide corresponding to amino acids 280-331 of drosophila SIAH1/SIAH2.
- Host:
- Mouse
- Reactivity:
- Fruit fly, Human, Pig, Rat, Zebra fish
- Specificity:
- This antibody is specific to human SIAH 1 and 2 and Drosophila SINA in proliferating cells and stem cells. This antibody does not detect Drosophila SINAH or normal, nondividing cells.
- Form:
- Liquid
- Isotype:
- IgG1
- Storage Buffer:
- In tissue culture supernatant (0.1% sodium azide)
- Storage Instruction:
- Store at 4°C. Do not freeze.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- Western Blot (1:500)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Inhibition of RAS-mediated transformation and tumorigenesis by targeting the downstream E3 ubiquitin ligase seven in absentia homologue.
Schmidt RL, Park CH, Ahmed AU, Gundelach JH, Reed NR, Cheng S, Knudsen BE, Tang AH.Cancer Res. 2007 Dec 15;67(24):11798-810.
- Applications
- Western Blot (Recombinant protein)
- SIAH1/SIAH2 monoclonal antibody, clone 8G7H12 (Cat # MAB2299) recognize both Drosophila SINA and human SIAH. There are two SINA-like E3 ligases, SINA and SINAH, in Drosophila.
SIAH1/SIAH2 monoclonal antibody, clone 8G7H12 is highly specific in recognizing both Drosophila SINA and human SIAH proteins but not Drosophila SINAH.
Coomassie blue staining patterns of both the wild-type (WT) and dominant negative (PD) forms of affinity-purified recombinant GST-SINA/SINAH/SIAH fusion proteins are shown in the top panel.
Protein standards were included and are shown in kDa.
Cross-reactivity of SIAH1/SIAH2 monoclonal antibody, clone 8G7H12 against Drosophila SINA and SINAH, and human hSIAH-1 and hSIAH-2 proteins were determined.
SIAH1/SIAH2 monoclonal antibody, clone 8G7H12 recognizes both human SIAH-1 and SIAH-2 protein.
Photo courtesy of Dr. Amy Tang, Mayo Clinic Cancer Center.