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Urokinase-type Plasminogen Activator ( uPA / PLAU ) Antibody
 
 
 
 
 
 
 
 
 
Order or Inquire for uPA Antibody product
 
 
 
 
 
 
 
Detect B chain and full-length of uPA in WB
 
 
 
 
 
 
Detection limit is 25 ng/lane in WB
 
 
 
 
 
 
uPA / PLAU抗体产品信息
 
 
 
 
免疫原 : 
 
 
Recombinant human uPA protein ( Catalog#10815-H08H )
 
 
 
 
抗体类型: 
 
Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
 
 
 
 
克隆号 :
 
 
 
2G11A6E10
 
 
 
 
抗体宿主 :
 
 
Mouse  IgG1
 
 
 
 
缓冲液 :
 
0.2 μm filtered solution in PBS with 5% trehalose
 
 
 
制备方法 : 
 
 
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human uPA / PLAU ( rhPLAU; Catalog#10815-H08H; Met 1-Leu 431; NP_002649.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
 
 
 
 
 
uPA / PLAU抗体使用指南
 
 
 
 
 
特异性 : 
 
 
 
Human uPA / PLAU
 
 
 
 
 
 
No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
 
 
 
Western blot :
 
This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect B chain and full-length of uPA in WB. Using a DAB  detection system, the detection limit for uPA is approximately 25 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended.
 
 
 
储存方法 :
 
This antibody can be stored at 2℃-8℃ for 1 month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
 Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
 
 
 
 
uPA / PLAU Antibody Related Products & Topics
 
 
相关研究领域:
 
Enzyme>>Protease & Regulator>>Serine Protease & Regulator>>Urokinase/PLAU/u-PA
 
Cancer>>Cancer Biomarkers>>Urokinase/PLAU/u-PA
 
Immunology>>Innate Immunity>>Coagulation>>Urokinase/PLAU/u-PA
 
蛋白:
 
 
 
 
Molecule
 
Species
 
Description //For Detailed Info.------CLICK!
 
Cat No
 
 
 
Urokinase/PLAU/u-PA
 
Human
 
Urokinase/PLAU/u-PA Protein, Recombinant
 
10815-H08H
 
 
 
 
抗体:
 
 
 
 
Molecule
 
Application
 
Description //For Detailed Info.------CLICK!
 
Cat No
 
 
 
Human Urokinase/PLAU/u-PA
 
WB
 
Mouse Monoclonal Antibody
 
10815-MM01
 
 
 
Human Urokinase/PLAU/u-PA
 
WB
 
Mouse Monoclonal Antibody
 
10815-MM02
 
 
 
Human Urokinase/PLAU/u-PA
 
ELISA
 
Mouse Monoclonal Antibody
 
10815-MM03
 
 
 
 
 
 uPA / PLAU抗体背景综述
 
 
Plasminogen activator, urokinase, also known as PLAU and uPA, is a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. The human PLAU is initially synthesized as 431 amino acid precursor with a N-terminal signal peptide (20 residues). The single chain molecule is processed into a disulfide-linked two-chain molecule of different molecular weights. Two forms of the A chain exist, starting at Ser21 (the long form) and Lys156 (the short form). The long and short A chains are unique to the high and low molecular weight forms, respectively. The long A chain contains an EGF-like domain, responsible for binding of the PLAU receptor. The B chain corresponds to the catalytic domain. PLAU is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium. A specific polymorphism in PLAU gene is implicated in late-onset Alzheimer disease and also with decreased affinity for fibrin-binding.
 
 
参考文献
 
 
 
Nagai, M. et al., 1985, Gene. 36:183-8.
 
Jacobs, P. et al., 1985, DNA. 4:139-46.
 
Geiger, M. et al., 1989,  Blood. 74 (2): 722-8.
 
Ploug, M. et al., 2002,  Biochem. Soc. Trans. 30 (2): 177-83. 
 
Duffy, MJ., 2002, Biochem. Soc. Trans. 30: 207-10.
 
Alfano, M. et al., 2004, J. Leukoc. Biol. 74 (5): 750-6.
 
 
 
 
 
 
 
 
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