Synonyms:	LCLC-103H（人大细胞肺癌）
Background:	established from the pleural effusion of a 61-year-old Caucasian man with large cell lung carcinoma with giant cells who had received chemo- and radiotherapy; described in the literature to be PAS negative, to exhibit remarkable stroma formation and to overexpress the proto-oncogene MYC
Species:	human (Homo sapiens)
Tissue:	lung
Disease:	large cell lung carcinoma
Gender:	N/A
Morphology:	pleomorphic, gigantic, adherent cells growing as monolayer; image
Growth Mode:	N/A
Doubling Time:	ca. 40 hours
DNA Profile:	N/A Cobioer’s Cell Line Authentication Service
Culture Medium:	RPMI-1640+10%FBS We strongly suggest to purchase the complete medium
Cryopreservation medium:	90%FBS+10%DMSO
Virus	ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Mycoplasma:	negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:	cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +
Fingerprint:	multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:	confirmed as human with IEF of AST, NP
Cytogenetics:	human, highly rearranged flat-moded hexaploid/heptaploid karyotype with 4% polyploidy - 130-160<7n>XXY, -X, -Y, -Y, +1, +1, -2, -2, -3, -6, -6, -8, -8, -9, -9, -9, -10, -13, -13, -13, -14, -14, +15, +16, +16, -17, -18, -19, -19, -20, -20, -20, -20, +18-25mar, del(1)(p22.2), add(1)(q11-21), i(1p)x2, add(3)(p23-24)x2, del(5)(q11q15), i(5q)x2, add(5)(p?), add(6)(q11)x2-3, add(7)(q34)x2, del(7)(q22)x2, i(8q), del(9)(p21)x2-3, del(10)(q23-24)x2, der(10;13)(q10;q10)x2, add(11)(q24-25)x2, del(11)(q23)x2, add(13)(p11), add(16)(q24) - variable number of dmin present - resembles published karyotype